| Field | Specification |
|---|---|
| Target | |
| Alternative names | Peptidyl-prolyl cis-trans isomerase FKBP8; PPIase FKBP8; 38 kDa FK506-binding protein; 38 kDa FKBP; FKBP-38; hFKBP38; FK506-binding protein 8; FKBP-8; FKBPR38; Rotamase; FKBP8; FKBP38 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FKBP38 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25F05 is a rabbit monoclonal antibody that recognizes peptidyl-prolyl cis-trans isomerase FKBP8 in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 44.6 kDa, and the supplier reports an observed band at about 52 kDa.
FKBP38 (FKBP8) is a membrane-anchored FKBP family protein found on mitochondria and the endoplasmic reticulum. Its isomerase activity is switched on by calcium and calmodulin, and it helps target the anti-apoptotic protein BCL2 to mitochondria and regulate apoptosis. FKBP8 is also studied in mitophagy and mTOR signaling.
| Target | Peptidyl-prolyl cis-trans isomerase FKBP8 (gene FKBP8; UniProt Q14318, human) |
|---|---|
| Clone | 25F05 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 44.6 kDa |
| Observed MW | 52 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human FKBP38.
Reactivity Notes
The supplier lists reactivity with human. UniProt places peptidyl-prolyl cis-trans isomerase FKBP8 in the mitochondrion, mitochondrion membrane and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
