| Field | Specification |
|---|---|
| Target | |
| Alternative names | Forkhead box protein P2;CAG repeat protein 44;Trinucleotide repeat-containing gene 10 protein;FOXP2;CAGH44, TNRC10; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human FOXP2 recombinant protein (Position: L637-E715). Human FOXP2 shares 100% amino acid (aa) sequence identity with both mouse and rat FOXP2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects forkhead box protein P2 (gene FOXP2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 79.9 kDa, and the supplier reports an observed band at about 90 kDa.
FOXP2 is a forkhead transcriptional repressor expressed in the developing brain, lung and gut. It is best known for its role in speech and language: mutations cause a rare inherited speech and language disorder, and the gene is studied in neural circuit development, vocal learning and language evolution.
| Target | Forkhead box protein P2 (gene FOXP2; UniProt O15409, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 79.9 kDa |
| Observed MW | 90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, human THP-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human FOXP2 fragment (Leu637–Glu715), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 100%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places forkhead box protein P2 in the nucleus. Tissue expression noted by UniProt (human): Isoform 1 and isoform 6 are expressed in adult and fetal brain, caudate nucleus and lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
