Anti-Frizzled 9 / CD349 Rabbit Monoclonal Antibody

SKU:BHA21009460
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Boster Bio
Boster Bio
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Overview
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Anti-FZD9 antibody (Rabbit monoclonal, clone 19F62, IgG). Validated for Western blot. Reactivity: Human, Mouse, Rat. Recognizes frizzled-9. Observed band in Western blot: about 64 kDa.
Target FZD9
clone number 19F62
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M08165 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB
      Application details: WB 1:500-2000<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target FZD9
Alternative names Frizzled-9; Fz-9; hFz9; FzE6; CD antigen CD349; FZD9; FZD3
Gene ID 8326
UniProt # O00144
Host Rabbit
Clone 19F62
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human Frizzled 9 / CD349
Molecular weight 64.5 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Secreted. Released from the secretory granules upon mast cell activation. .
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M08165
Main SKU BHA21009460
Rabbit Monoclonal

Product Overview

Clone 19F62 is a rabbit monoclonal antibody that recognizes frizzled-9 (gene FZD9) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 64.5 kDa, and the supplier reports an observed band at about 64 kDa.

Frizzled-9 (FZD9) is a Wnt receptor of the frizzled family expressed in brain, testis and other tissues. It has been linked to neural development, hippocampal function, bone formation and B-cell development. FZD9 lies in the region deleted in Williams syndrome.

Target Frizzled-9 (gene FZD9; UniProt O00144, human)
Clone 19F62
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 64.5 kDa
Observed MW 64 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Samples with a confirmed band (WB): human Seminoma.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human frizzled 9 / CD349.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places frizzled-9 in the cell membrane. Tissue expression noted by UniProt (human): Expressed predominantly in adult and fetal brain, testis, eye, skeletal muscle and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 64.5 kDa; observed band at about 64 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human frizzled 9 / CD349.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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