| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Alpha-synuclein;Non-A beta component of AD amyloid;Non-A4 component of amyloid precursor;NACP;SNCA;NACP, PARK1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human FTO Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-FTO antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AEAA-6; isotype Rabbit IgG; reactivity: Human,Rat. Reported application contexts include WB, IHC, ICC, IF (as provided in the source record). Boster Bio Anti-FTO Monoclonal Antibody catalog # M00219. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Rat.
Key elements and design rationale
- Target: FTO (Alpha-synuclein).
- Antibody format: Monoclonal; clone AEAA-6; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
FTO (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. Reported cellular localization context: Cytoplasm, cytosol . Membrane . Nucleus . Cell junction, synapse . Secreted . Membrane-bound in dopaminergic neurons. . Tissue expression notes (as provided): Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
Research relevance and current trends
- Research context keywords from the source record include: Atherosclerosis,Cancer,Cardiovascular,Diabetes,Diabetes-associated,Heart Disease,Lipid and Lipoprotein Metabolism,Metabolic Signaling Pathways,Metabolism,Neurology Process,Neuroscience,Obesity,Pathways and Processes.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
Workflow ideas (metafield): Validate FTO antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect FTO expression by Western blot in cell or tissue lysates, Detect FTO in FFPE tissue sections by immunohistochemistry, Localize FTO by immunofluorescence/immunocytochemistry in cultured cells
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 58 kDa; calculated MW: 14460 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 58 kDa
- Cellular localization (provided): Cytoplasm, cytosol . Membrane . Nucleus . Cell junction, synapse . Secreted . Membrane-bound in dopaminergic neurons. .
- Tissue details (provided): Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
