| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Phospholemman;FXYD domain-containing ion transport regulator 1;FXYD1;PLM; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human FXYD1 recombinant protein (Position: E21-R92). Human FXYD1 shares 86.1% and 87.5% amino acid (aa) sequence identity with mouse and rat FXYD1, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of FXYD1 (Phospholemman) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-FXYD1 Antibody Picoband® catalog # PB9704. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human FXYD1 recombinant protein (Position: E21-R92). Human FXYD1 shares 86.1% and 87.5% amino acid (aa) sequence identity with mouse and rat FXYD1, respectively. (reported region: E21-R92).
- Molecular weight context: reported MW: 10 kDa; calculated MW: 10441 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Phospholemman; Phospholemman. Phospholemman?is a?protein?that in humans is encoded by the?FXYD1?gene. This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. Transcript variants with different 5' UTR sequences have been described in the literature. Functional note: May have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed. Reported localization: Membrane; Single-pass type I membrane protein. Expression/tissue context: Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, prostate without endothelial lining, spleen, and testis.
Research relevance and current trends
- ATPases: Researchers commonly examine how FXYD1 (Phospholemman) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how FXYD1 (Phospholemman) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how FXYD1 (Phospholemman) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative FXYD1 (Phospholemman) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of FXYD1 (Phospholemman) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
