| Field | Specification |
|---|---|
| Target | |
| Alternative names | Granulocyte colony-stimulating factor;G-CSF;Pluripoietin;Filgrastim;Lenograstim;CSF3;C17orf33, GCSF; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human G-CSF |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABAC-3 is a rabbit monoclonal antibody that recognizes granulocyte colony-stimulating factor (gene CSF3) in human, mouse and rat samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 22.3 kDa, and the supplier reports an observed band at about 25 kDa.
G-CSF (granulocyte colony-stimulating factor, gene CSF3) is the main cytokine that drives the production, maturation and release of neutrophils from the bone marrow. It is measured in studies of infection, inflammation and bone marrow recovery, and recombinant G-CSF is used clinically to treat neutropenia.
| Target | Granulocyte colony-stimulating factor (gene CSF3; UniProt P09919, human) |
|---|---|
| Clone | ABAC-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 22.3 kDa |
| Observed MW | 25 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human G-CSF.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes granulocyte colony-stimulating factor as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Balamayooran Theivanthiran et al. (2022) Tumor-intrinsic NLRP3-HSP70-TLR4 axis drives premetastatic niche development and hyperprogression during anti–PD-1 immunotherapy. Science Translational Medicine. 10.1126/scitranslmed.abq7019
Zheng-Xi Li et al. (2009) Granulocyte-colony stimulating factor is involved in low-dose LPS-induced neuroprotection. Neuroscience Letters. 10.1016/j.neulet.2009.08.069
