| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | γ-Aminobutyric acid type B receptor subunit 2, GABA B receptor 2, GABABR2, Gb2, GABBR2, GPR51, GPRC3B, HG20 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
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| Target |
Overview
Anti-GABA(B) R2 Antibody is an antibody targeting γ-Aminobutyric acid type B receptor subunit 2, GABA B receptor 2, GABABR2, Gb2, GABBR2, GPR51, GPRC3B, HG20 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IF, IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: γ-Aminobutyric acid type B receptor subunit 2, GABA B receptor 2, GABABR2, Gb2, GABBR2, GPR51, GPRC3B, HG20 (also reported as γ-Aminobutyric acid type B receptor subunit 2, GABA B receptor 2, GABABR2, Gb2, GABBR2, GPR51, GPRC3B, HG20).
- Immunogen/epitope region: Intracellular, C-terminus.
- Homology note: Rat, mouse, bovine - identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Cited use: IP (literature use does not guarantee performance in every setup).
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.1,2The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA(A) R and GABA(C) R, and the metabotropic GABA(B) receptor, [GABA(B) R].3 The latter belongs to the G-protein coupled receptor (GPCR) superfamily and mediates slow synaptic inhibition in the brain and spinal cord.1,4The functional GABA(B) receptor is a heterodimer consisting of two subunits, the GABA(B) R1 and the GABA(B) R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.5,6GABA(B) R2 has been shown to be essential for trafficking and for G-protein binding while the GABA(B) R1 subunit has been demonstrated to be important for agonist and antagonist binding. GABA(B) R2 is highly expressed in the brain, especially in the cerebral cortex, thalamus, and hippocampus, spinal cord, and, to a lesser extent, in the testis.7
Research relevance and current trends
- Mapping receptor/channel localization across neuronal subtypes and subcellular compartments.
- Linking trafficking or surface expression changes to activity-dependent signaling and plasticity.
- Using KO/KD or blocking-peptide concepts to strengthen antibody-based target assignment.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-GB002.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-GB002; Negative control: BLP-GB002.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
