| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Glutamate decarboxylase 1;4.1.1.15;67 kDa glutamic acid decarboxylase;GAD-67;Glutamate decarboxylase 67 kDa isoform;GAD1;GAD, GAD67; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human GAD67 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-GAD1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AAGO-7; isotype Rabbit IgG; reactivity: Human,Mouse,Rat,Pig. Reported application contexts include WB, IHC, IP (as provided in the source record). Boster Bio Anti-GAD67 GAD1 Rabbit Monoclonal Antibody catalog # M02002. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat, Pig.
Key elements and design rationale
- Target: GAD1 (Glutamate decarboxylase 1).
- Antibody format: Monoclonal; clone AAGO-7; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat,Pig (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
GAD1 (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Catalyzes the production of GABA. Reported cellular localization context: Nucleus . RORG promotes its nuclear localization. . Tissue expression notes (as provided): Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. .
Research relevance and current trends
- Research context keywords from the source record include: Amino Acid Metabolism,Amino Acids,Autoimmune,Cancer,Immune System Diseases,Immunology,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate GAD1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect GAD1 expression by Western blot in cell or tissue lysates, Detect GAD1 in FFPE tissue sections by immunohistochemistry, Enrich GAD1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 70 kDa; calculated MW: 66897 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 70 kDa
- Cellular localization (provided): Nucleus . RORG promotes its nuclear localization. .
- Tissue details (provided): Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
