| Field | Specification |
|---|---|
| Target | |
| Alternative names | Growth arrest and DNA damage-inducible protein GADD45 alpha;DNA damage-inducible transcript 1 protein;DDIT-1;GADD45A;DDIT1, GADD45; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human GADD45A, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes growth arrest and DNA damage-inducible protein GADD45 alpha (gene GADD45A) in human samples and is validated for Western blot. The predicted molecular weight is 18.3 kDa, and the supplier reports an observed band at about 19 kDa.
GADD45A is a small nuclear protein induced by DNA damage and other stresses, in part through p53. It helps arrest the cell cycle at the G2/M transition, interacts with PCNA and supports DNA repair, and can activate p38 and JNK stress kinase pathways through MEKK4. It is studied in genome stability, apoptosis and cancer.
| Target | Growth arrest and DNA damage-inducible protein GADD45 alpha (gene GADD45A; UniProt P24522, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 18.3 kDa |
| Observed MW | 19 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human GADD45A. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human. UniProt places growth arrest and DNA damage-inducible protein GADD45 alpha in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yang Meng et al. (2020) An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy. Journal of Nanobiotechnology. 10.1186/s12951-020-00656-9
