| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Galectin-3;Gal-3;35 kDa lectin;Carbohydrate-binding protein 35;CBP 35;Galactose-specific lectin 3;Galactoside-binding protein;GALBP;IgE-binding protein;L-31;Laminin-binding protein;Lectin L-29;Mac-2 antigen;LGALS3;MAC2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Galectin 3 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-LGALS3 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AOIE-12; isotype Rabbit IgG; reactivity: Human,Rat. Reported application contexts include WB, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-Galectin 3 LGALS3 Rabbit Monoclonal Antibody catalog # M00621. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Rat.
Key elements and design rationale
- Target: LGALS3 (Galectin-3).
- Antibody format: Monoclonal; clone AOIE-12; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
LGALS3 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. . Reported cellular localization context: Cytoplasm. Nucleus. Secreted. Secreted by a non-classical secretory pathway and associates with the cell surface. Tissue expression notes (as provided): A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. .
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Cell Cycle,Cell Division,Oncoproteins,Tumor Biomarkers.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate LGALS3 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect LGALS3 expression by Western blot in cell or tissue lysates, Localize LGALS3 by immunofluorescence/immunocytochemistry in cultured cells, Quantify LGALS3-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 29 kDa; calculated MW: 26152 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 29 kDa
- Cellular localization (provided): Cytoplasm. Nucleus. Secreted. Secreted by a non-classical secretory pathway and associates with the cell surface.
- Tissue details (provided): A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
