| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Transcription factor GATA-5;GATA-binding factor 5;GATA5 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human GATA5 recombinant protein (Position: M1-A397). Human GATA5 shares 75% amino acid (aa) sequence identity with mouse GATA5. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of GATA5 (Transcription factor GATA-5) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-GATA5 Antibody Picoband® catalog # PB9200. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human GATA5 recombinant protein (Position: M1-A397). Human GATA5 shares 75% amino acid (aa) sequence identity with mouse GATA5. (reported region: M1-A397).
- Molecular weight context: reported MW: 50 kDa; calculated MW: 41299 MW
- Reactivity: Human
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Transcription factor GATA-5; Transcription factor GATA-5. Transcription factor GATA-5, also named GATAS or GATA5, is a protein that in humans is encoded by the GATA5 gene. It is mapped to 20q13.33. The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity. In addition, the expression of GATA5 increased in a human colon cancer cell line within 2 hours of exposure to sodium butyrate, which induces differentiation. Functional note: Transcription factor required during cardiovascular development (PubMed:23289003). Plays an important role in the transcriptional program (s) that underlies smooth muscle cell diversity (By similarity). Binds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer (PubMed:25543888). . Reported localization: Nucleus . Expression/tissue context: T-cells and endothelial cells.
Research relevance and current trends
- Domain Families: Researchers commonly examine how GATA5 (Transcription factor GATA-5) relates to this theme using model systems and orthogonal readouts.
- Epigenetics and Nuclear Signaling: Researchers commonly examine how GATA5 (Transcription factor GATA-5) relates to this theme using model systems and orthogonal readouts.
- Transcription: Researchers commonly examine how GATA5 (Transcription factor GATA-5) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative GATA5 (Transcription factor GATA-5) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Contains 2 GATA-type zinc fingers.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
