| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Glucose-6-phosphate 1-dehydrogenase;G6PD;1.1.1.49;G6PD; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human G6PD recombinant protein (Position: E315-L515). Human G6PD shares 95% and 96% amino acid (aa) sequences identity with mouse and rat G6PD, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of G6PD (Glucose-6-phosphate 1-dehydrogenase) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Glucose 6 Phosphate Dehydrogenase/G6PD Antibody Picoband® catalog # PB9198. Tested in WB applications. This antibody reacts with Human;Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human G6PD recombinant protein (Position: E315-L515). Human G6PD shares 95% and 96% amino acid (aa) sequences identity with mouse and rat G6PD, respectively. (reported region: E315-L515).
- Molecular weight context: reported MW: 59 kDa; calculated MW: 59257 MW
- Reactivity: Human,Mouse
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Glucose-6-phosphate 1-dehydrogenase; Glucose-6-phosphate 1-dehydrogenase. Glucose-6-phosphate dehydrogenase, also known as G6PD or G6PDH, is an enzyme that in humans is encoded by the G6PD gene. It is mapped to Xq28. G6PD plays a key role in the production of ribose 5-phosphate and the generation of NADPH in the hexose monophosphate pathway. Because this pathway is the only NADPH-generation process in mature red cells, which lack the citric adid cycle, a genetic deficiency of G6PD is often associated with adverse physiologic effects. It has been found that aldosterone decreased G6PD expression and activity, resulting in increased oxidant stress and decreased nitric oxide levels, similar to what is observed in G6PD-deficient endothelial cells. Functional note: Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. . Reported localization: Cytoplasm, cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. Expression/tissue context: Isoform Long is found in lymphoblasts, granulocytes and sperm.
Research relevance and current trends
- Atherosclerosis: Researchers commonly examine how G6PD (Glucose-6-phosphate 1-dehydrogenase) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how G6PD (Glucose-6-phosphate 1-dehydrogenase) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how G6PD (Glucose-6-phosphate 1-dehydrogenase) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative G6PD (Glucose-6-phosphate 1-dehydrogenase) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the glucose-6-phosphate dehydrogenase family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
