| Field | Specification |
|---|---|
| Target | |
| Alternative names | Guanine nucleotide-binding protein G; i; subunit alpha-2; Adenylate cyclase-inhibiting G alpha protein; GNAI2; GNAI2B |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GNAI2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29G37 is a rabbit monoclonal antibody that recognizes guanine nucleotide-binding protein G(i) subunit alpha-2 (gene GNAI2) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 40.5 kDa, and the supplier reports an observed band at about 40 kDa.
Gαi2 (GNAI2) is the alpha subunit of an inhibitory G protein. When activated by G protein-coupled receptors it exchanges GDP for GTP and inhibits adenylyl cyclase, lowering cAMP, and its GTPase activity then turns the signal off. It is studied in immune cell migration, cardiac signaling and hormone regulation.
| Target | Guanine nucleotide-binding protein G(i) subunit alpha-2 (gene GNAI2; UniProt P04899, human) |
|---|---|
| Clone | 29G37 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 40.5 kDa |
| Observed MW | 40 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): U-87 MG cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human GNAI2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places guanine nucleotide-binding protein G(i) subunit alpha-2 in the cytoplasm, cell membrane and membrane. Tissue expression noted by UniProt (human): Highly expressed in endothelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
