| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Adhesion G-Protein Coupled Receptor G6, G-Protein Coupled Receptor 126, Developmentally Regulated G-Protein-Coupled Receptor, DREG, Vascular Inducible G Protein-Coupled Receptor, VIGR |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
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| Target |
Overview
Anti-GPR126/ADGRG6 (extracellular) Antibody is an antibody targeting Adhesion G-Protein Coupled Receptor G6, G-Protein Coupled Receptor 126, Developmentally Regulated G-Protein-Coupled Receptor, DREG, Vascular Inducible G Protein-Coupled Receptor, VIGR Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Adhesion G-Protein Coupled Receptor G6, G-Protein Coupled Receptor 126, Developmentally Regulated G-Protein-Coupled Receptor, DREG, Vascular Inducible G Protein-Coupled Receptor, VIGR (also reported as Adhesion G-Protein Coupled Receptor G6, G-Protein Coupled Receptor 126, Developmentally Regulated G-Protein-Coupled Receptor, DREG, Vascular Inducible G Protein-Coupled Receptor, VIGR).
- Immunogen/epitope region: Extracellular, N-terminus.
- Homology note: Rat, human - identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
GPR126 is an adhesion G-protein coupled receptor; the second largest subgroup of GPCRs1. The defining characteristic of the adhesion GPCR family is their extraordinarily large N-terminal extracellular domain important to communicate with extracellular ligands and mediate adhesive functions2.GPR126 is implicated in an increasing number of developmental defects: skeletal defects, including shortened height, adolescent idiopathic scoliosis, arthrogryposis multiplex congenital, and periodontitis. Other studies reveal that GPR126 is essential for myelination of axons in the peripheral nervous system and the heart2.Known ligands of GPCR126 are type IV collagen (COLIV) in peripheral nerves and inner ear development, Laminin-211 during Schwann cell development, and prion protein in myelination3, suggesting that GPR126 could use different signaling pathways in different tissues4.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-GR056.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-GR056; Negative control: BLP-GR056.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
