| Field | Specification |
|---|---|
| Target | |
| Alternative names | G protein-coupled receptor kinase 6;2.7.11.16;G protein-coupled receptor kinase GRK6;GRK6;GPRK6; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human GRK6, different from the related mouse sequence by four amino acids, and from the related rat sequence by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets G protein-coupled receptor kinase 6 (gene GRK6) in human samples and is validated for Western blot. The predicted molecular weight is 66.0 kDa, and the supplier reports an observed band at about 66 kDa.
GRK6 is a G protein-coupled receptor kinase that phosphorylates activated receptors so that β-arrestins can bind and dampen signaling. It helps desensitize D2-like dopamine receptors in the striatum and the chemokine receptor CXCR4, which shapes cell migration toward CXCL12. GRK6 is studied in dopamine signaling, immune cell chemotaxis and receptor regulation.
| Target | G protein-coupled receptor kinase 6 (gene GRK6; UniProt P43250, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 66.0 kDa |
| Observed MW | 66 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human GRK6. It differs from the mouse sequence at four residues and from the rat sequence at three residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places G protein-coupled receptor kinase 6 in the membrane. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Mona Reichel et al. (2022) Suitability of GRK Antibodies for Individual Detection and Quantification of GRK Isoforms in Western Blots. International Journal of Molecular Sciences. 10.3390/ijms23031195
