| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Glycogen synthase kinase-3 beta;GSK-3 beta;2.7.11.26;Xgsk-3 protein;gsk3b;Xgsk-3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human GSK3 beta |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-GSK3 beta Rabbit Monoclonal Antibody is an antibody targeting GSK3B. Common applications include WB, IHC, ICC, IF, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: EB-7; isotype: Rabbit IgG; reactivity: Human,Mouse; observed MW: 47 kDa; calculated MW: 47 kDa.
Boster Bio Anti-GSK3 beta Rabbit Monoclonal Antibody catalog # M00791. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: GSK3B — Glycogen synthase kinase-3 beta
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: EB-7; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse
- Molecular weight guidance: Observed: 47 kDa; Calculated: 47 kDa
Biological background
Protein function (datasheet): Plays a role in the organization of the formation of the main body axis of developing embryo. Acts as an inhibitor of differentiation of primary neurons. Inhibits the ability of ectopically expressed NEUROD1 and other bHLH factors to promote early retinal cell differentiation. May participate in the Wnt signaling pathway. May regulate the circadian clock via phosphorylation of the major clock components. .
Cellular localization (datasheet): Cytoplasm . Nucleus . Cell membrane .
Tissue details (datasheet): Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain.
Research relevance and current trends
- Commonly studied in contexts related to Carbohydrate Metabolism,Cell Biology,Cell Cycle,Cell Differentiation,Developmental Biology,Energy Metabolism,Metabolic Signaling Pathways,Metabolism,Nervous System Development,Neurogenesis,Neurology Process,Neuroscience,Organogenesis,Pathways and Processes,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
