| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glycogen synthase kinase-3 beta;GSK-3 beta;2.7.11.26;Xgsk-3 protein;gsk3b;Xgsk-3; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human GSK3 beta |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EB-7 is a rabbit monoclonal antibody that recognizes glycogen synthase kinase-3 beta (gene GSK3B) in human and mouse samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 46.7 kDa, and the supplier reports an observed band at about 46 kDa.
Glycogen synthase kinase-3 beta (GSK3B) is a widely expressed, constitutively active serine/threonine kinase that is inhibited by insulin, growth factor and Wnt signals. Its substrates include glycogen synthase, β-catenin, tau and several transcription factors, placing it at the crossroads of metabolism, Wnt signaling and cytoskeletal regulation. GSK3B is heavily studied in diabetes, neurodegeneration, mood disorders and cancer.
| Target | Glycogen synthase kinase-3 beta (gene GSK3B; UniProt P49841, human) |
|---|---|
| Clone | EB-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 46.7 kDa |
| Observed MW | 46 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:200–1:500 |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, human U87 cells, human MCF-7 cells, rat PC-12 cells, rat C6 cells, mouse RAW264.7 cells, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue, human prostate cancer tissue, human small cells lung cancer tissue, human pancreatic cancer tissue, human colon carcinome tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human GSK3 beta.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places glycogen synthase kinase-3 beta in the cytoplasm, nucleus and cell membrane. Tissue expression noted by UniProt (human): Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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