| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Basement membrane-specific heparan sulfate proteoglycan core protein;HSPG;Perlecan;PLC;Endorepellin;LG3 peptide;HSPG2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human HSPG2 recombinant protein (Position: F524-K701). Human HSPG2 shares 86% amino acid (aa) sequence identity with mouse HSPG2. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of HSPG2 (Basement membrane-specific heparan sulfate proteoglycan core protein) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband® catalog # PB9277. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human HSPG2 recombinant protein (Position: F524-K701). Human HSPG2 shares 86% amino acid (aa) sequence identity with mouse HSPG2. (reported region: F524-K701).
- Molecular weight context: reported MW: 469 kDa; calculated MW: 468830 MW
- Reactivity: Human
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Basement membrane-specific heparan sulfate proteoglycan core protein; Basement membrane-specific heparan sulfate proteoglycan core protein. Perlecan (PLC) also known as HSPG2, is a protein that in humans is encoded by the HSPG2 gene. It is mapped to 1p36.12. Perlecan is highly conserved across species and the available data indicate that it has evolved from ancient ancestors by gene duplication and exonshuffling. Perlecan is a key component of the vascular extracellular matrix, here it interacts with a variety of other matrix components and helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. Perlecan can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation. Functional note: Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development. Reported localization: Secreted, extracellular space, extracellular matrix, basement membrane. Expression/tissue context: Found in the basement membranes.
Research relevance and current trends
- Angiogenesis: Researchers commonly examine how HSPG2 (Basement membrane-specific heparan sulfate proteoglycan core protein) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how HSPG2 (Basement membrane-specific heparan sulfate proteoglycan core protein) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how HSPG2 (Basement membrane-specific heparan sulfate proteoglycan core protein) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative HSPG2 (Basement membrane-specific heparan sulfate proteoglycan core protein) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of HSPG2 (Basement membrane-specific heparan sulfate proteoglycan core protein) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Contains 4 EGF-like domains.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
