| Field | Specification |
|---|---|
| Target | |
| Alternative names | Basement membrane-specific heparan sulfate proteoglycan core protein;HSPG;Perlecan;PLC;Endorepellin;LG3 peptide;HSPG2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human HSPG2 recombinant protein (Position: F524-K701). Human HSPG2 shares 86% amino acid (aa) sequence identity with mouse HSPG2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects basement membrane-specific heparan sulfate proteoglycan core protein (gene HSPG2) in human samples and is validated for IHC and Western blot. The predicted molecular weight is 468.8 kDa, and the supplier reports an observed band at about 469 kDa.
Perlecan (HSPG2) is a large heparan sulfate proteoglycan and a core component of basement membranes. It binds growth factors and other matrix proteins, contributes to the filtering barrier of the kidney glomerulus and supports cartilage and vascular development. HSPG2 mutations cause Schwartz-Jampel syndrome and dyssegmental dysplasia.
| Target | Basement membrane-specific heparan sulfate proteoglycan core protein (gene HSPG2; UniProt P98160, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 468.8 kDa |
| Observed MW | 469 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): human Caco-2 cells, human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant human HSPG2 fragment (Phe524–Lys701), expressed in E. coli. Sequence identity with the mouse ortholog: 86%.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes basement membrane-specific heparan sulfate proteoglycan core protein as a secreted protein. Tissue expression noted by UniProt (human): Detected in cerebrospinal fluid, fibroblasts and urine (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tomáš Vaisar et al. (2020) Parallel Murine and Human Plaque Proteomics Reveals Pathways of Plaque Rupture. Circulation Research. 10.1161/CIRCRESAHA.120.317295
Jakara Griffin et al. (2022) Red Light Mitigates the Deteriorating Placental Extracellular Matrix in Late Onset of Preeclampsia and Improves the Trophoblast Behavior. Journal of Pregnancy. 10.1155/2022/3922368
