Anti-HLA F Rabbit Monoclonal Antibody

Overview

This product is an anti-HLA-F antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 25H28; isotype IgG; reactivity: Human. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-HLA F Rabbit Monoclonal Antibody catalog # M04798-1. Tested in WB, IHC applications. This antibody reacts with Human.

Key elements and design rationale

• Target: HLA-F (Alpha-sarcoglycan).
• Antibody format: Monoclonal; clone 25H28; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

HLA-F (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. Reported cellular localization context: Cell membrane, sarcolemma ; Single-pass type I membrane protein . Cytoplasm, cytoskeleton . Tissue expression notes (as provided): Most strongly expressed in skeletal muscle. Also expressed in cardiac muscle and, at much lower levels, in lung. In the fetus, most abundant in cardiac muscle and, at lower levels, in lung. Also detected in liver and kidney. Not expressed in brain.

Research relevance and current trends

• Research context keywords from the source record include: Cancer,Cancer Metabolism,Carbohydrate Metabolism,Cardiovascular,Cholesterol Metabolism,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism Of Carbohydrates,Mitochondrial,Mitochondrial Markers,Mitochondrial Metabolism,Obesity,Pathways and Processes,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.

Workflow ideas (metafield): Validate HLA-F antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect HLA-F expression by Western blot in cell or tissue lysates, Detect HLA-F in FFPE tissue sections by immunohistochemistry

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 42 kDa; calculated MW: 42875 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 42 kDa
• Cellular localization (provided): Cell membrane, sarcolemma ; Single-pass type I membrane protein . Cytoplasm, cytoskeleton .
• Tissue details (provided): Most strongly expressed in skeletal muscle. Also expressed in cardiac muscle and, at much lower levels, in lung. In the fetus, most abundant in cardiac muscle and, at lower levels, in lung. Also detected in liver and kidney. Not expressed in brain.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.