| Field | Specification |
|---|---|
| Target | |
| Alternative names | 3-hydroxy-3-methylglutaryl-coenzyme A reductase;HMG-CoA reductase;1.1.1.34;HMGCR; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HMGCR |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOCC-8 is a rabbit monoclonal antibody that recognizes 3-hydroxy-3-methylglutaryl-coenzyme A reductase (gene HMGCR) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 97.5 kDa, and the supplier reports an observed band at about 97 kDa.
HMG-CoA reductase (HMGCR) is the endoplasmic reticulum enzyme that converts HMG-CoA to mevalonate, the rate-limiting step of the mevalonate pathway that makes cholesterol and other isoprenoids. It is tightly regulated by sterol levels and is the target of statin drugs, which makes it central to cholesterol metabolism research.
| Target | 3-hydroxy-3-methylglutaryl-coenzyme A reductase (gene HMGCR; UniProt P04035, human) |
|---|---|
| Clone | AOCC-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 97.5 kDa |
| Observed MW | 97 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human HMGCR.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 3-hydroxy-3-methylglutaryl-coenzyme A reductase in the endoplasmic reticulum membrane and peroxisome membrane. Tissue expression noted by UniProt (human): Ubiquitously expressed with the highest levels in the cerebellum, fetal brain, testis, skin and adrenal gland... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qiu Fan et al. (2019) Metabolic effects of mulberry branch bark powder on diabetic mice based on GC-MS metabolomics approach. Nutrition & Metabolism. 10.1186/s12986-019-0335-x
