| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 3-hydroxy-3-methylglutaryl-coenzyme A reductase;HMG-CoA reductase;1.1.1.34;HMGCR; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human HMGCR |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-HMGCR antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AOCC-8; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IP (as provided in the source record). Boster Bio Anti-HMGCR/Hmg Coa Reductase Rabbit Monoclonal Antibody catalog # M00643. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: HMGCR (3-hydroxy-3-methylglutaryl-coenzyme A reductase).
- Antibody format: Monoclonal; clone AOCC-8; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
HMGCR (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins. Reported cellular localization context: Endoplasmic reticulum membrane; Multi-pass membrane protein. Tissue expression notes (as provided): Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
Research relevance and current trends
- Research context keywords from the source record include: Atherosclerosis,Cancer,Cancer Metabolism,Cardiovascular,Cholesterol Metabolism,Heart Disease,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism of Lipids and Lipoproteins,Pathways and Processes,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate HMGCR antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect HMGCR expression by Western blot in cell or tissue lysates, Enrich HMGCR by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 85 kDa; calculated MW: 97476 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 85 kDa
- Cellular localization (provided): Endoplasmic reticulum membrane; Multi-pass membrane protein.
- Tissue details (provided): Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
