| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heterogeneous nuclear ribonucleoprotein M; hnRNP M; HNRNPM; HNRPM; NAGR1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HNRNPM |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29H77 is a rabbit monoclonal antibody that recognizes heterogeneous nuclear ribonucleoprotein M (gene HNRNPM) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 77.5 kDa, and the supplier reports an observed band at about 73 kDa.
hnRNP M (HNRNPM) is a pre-mRNA-binding protein that associates with the spliceosome and regulates alternative splicing. On liver Kupffer cells it can also act as a binding site for carcinoembryonic antigen, and it is studied in RNA processing and tumor progression.
| Target | Heterogeneous nuclear ribonucleoprotein M (gene HNRNPM; UniProt P52272, human) |
|---|---|
| Clone | 29H77 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 77.5 kDa |
| Observed MW | 73 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human HepG2 cells, human MCF-7 cells, rat liver tissue, rat lung tissue, mouse liver tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human rectal cancer tissue, human stomach cancer tissue, mouse liver tissue, rat liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human HNRNPM.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places heterogeneous nuclear ribonucleoprotein M in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xin Pan et al. (2025) Tumor Microenvironment T-cell Heterogeneity and Prognostic Model Construction in Laryngeal Squamous Cell Carcinoma by Single-cell and Bulk RNA Sequencing. Computer Methods in Biomechanics and Biomedical Engineering. 10.1080/10255842.2025.2570515
