Anti-HSD11B2 Antibody Picoband®

Overview

This antibody is intended for detection of HSD11B2 (Corticosteroid 11-beta-dehydrogenase isozyme 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-HSD11B2 Antibody catalog # RP1094. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human HSD11B2, different from the related mouse sequence by five amino acids, and from the related rat sequence by three amino acids.
• Molecular weight context: reported MW: 43 kDa; calculated MW: 44127 MW
• Reactivity: Human,Mouse,Rat
• Applications: IF, IHC, IHC-F, WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

Corticosteroid 11-beta-dehydrogenase isozyme 2; Corticosteroid 11-beta-dehydrogenase isozyme 2. Corticosteroid 11-β-dehydrogenase isozyme 2, also known as?11-β-hydroxysteroid dehydrogenase 2,?is an enzyme?that in humans is encoded by the?HSD11B2?gene. There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension. Functional note: Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids. Reported localization: Microsome . Endoplasmic reticulum . Expression/tissue context: Expressed in kidney, pancreas, prostate, ovary, small intestine and colon. At midgestation, expressed at high levels in placenta and in fetal kidney and, at much lower levels, in fetal lung and testis (PubMed:8530071). .

Research relevance and current trends

• Atherosclerosis: Researchers commonly examine how HSD11B2 (Corticosteroid 11-beta-dehydrogenase isozyme 2) relates to this theme using model systems and orthogonal readouts.
• Cancer: Researchers commonly examine how HSD11B2 (Corticosteroid 11-beta-dehydrogenase isozyme 2) relates to this theme using model systems and orthogonal readouts.
• Cancer Metabolism: Researchers commonly examine how HSD11B2 (Corticosteroid 11-beta-dehydrogenase isozyme 2) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative HSD11B2 (Corticosteroid 11-beta-dehydrogenase isozyme 2) levels across conditions; band patterns may reflect isoforms and processing.
• IHC/IHC-F: assess spatial distribution of HSD11B2 (Corticosteroid 11-beta-dehydrogenase isozyme 2) across tissue regions and cell types using matched controls.
• IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.