| Field | Specification |
|---|---|
| Target | |
| Alternative names | Corticosteroid 11-beta-dehydrogenase isozyme 2;1.1.1.-;11-beta-hydroxysteroid dehydrogenase type 2;11-DH2;11-beta-HSD2;11-beta-hydroxysteroid dehydrogenase type II;11-HSD type II;11-beta-HSD type II;NAD-dependent 11-beta-hydroxysteroid dehydrogenase;11-beta-HSD;Short chain dehydrogenase/reductase family 9C member 3;HSD11B2;HSD11K , SDR9C3; |
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| Clonality | |
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| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human HSD11B2, different from the related mouse sequence by five amino acids, and from the related rat sequence by three amino acids. |
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| Reconstitution | |
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| Form | Lyophilized |
| Storage | |
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Product Overview
This rabbit polyclonal antibody targets 11-beta-hydroxysteroid dehydrogenase type 2 (gene HSD11B2) in human, mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 44.1 kDa, and the supplier reports an observed band at about 43 kDa.
11β-hydroxysteroid dehydrogenase type 2 (HSD11B2) uses NAD+ to convert active cortisol into inactive cortisone. In the kidney this protects the mineralocorticoid receptor from cortisol, and in the placenta it limits fetal exposure to maternal glucocorticoids. Its loss causes the syndrome of apparent mineralocorticoid excess.
| Target | 11-beta-hydroxysteroid dehydrogenase type 2 (gene HSD11B2; UniProt P80365, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 44.1 kDa |
| Observed MW | 43 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| IHC (frozen sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): rat kidney tissue, mouse kidney tissue, human placenta tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse pancreas tissue, rat pancreas tissue, human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (IHC): human placenta tissue, mouse kidney tissue, rat kidney tissue.
Samples with confirmed staining (ICC/IF): human placenta tissue.
Immunogen
Synthetic peptide from the C-terminal region of human HSD11B2. It differs from the mouse sequence at five residues and from the rat sequence at three residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 11-beta-hydroxysteroid dehydrogenase type 2 in the microsome and endoplasmic reticulum. Tissue expression noted by UniProt (human): Expressed in kidney, placenta, pancreas, prostate, ovary, small intestine and colon, and in lower levels in the spleen and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Gao Rui et al. (2025) Bisphenol A disrupts renal metabolism via MAPK-mediated steroid hormone dysregulation identified by transcriptomic and metabolomic analyses. Scientific Reports. 10.1038/s41598-025-20141-3
Juan Yuan et al. (2025) Propofol induces mitochondrial dysfunction in hippocampal neurons and postoperative cognitive dysfunction in male 3xTg-AD mice by blocking ESRRG-mediated HSD11B2 transcription. Brain Research. 10.1016/j.brainres.2025.150035
Lin Wang et al. (2013) Expression of 11β-HSD in steroid-induced avascular necrosis of the femoral head. Molecular Medicine Reports. 10.3892/mmr.2013.1401
