| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Heat shock 70 kDa protein 1B;Heat shock 70 kDa protein 1;HSP70.1;Hspa1b;Hcp70.1, Hsp70-1, Hsp70a1, Hspa1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | HSP70 isolated from bovine brain. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22) is an antibody targeting HSPA1A; HSPA1B. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: BRM-22; isotype: Mouse IgG1; reactivity: Bovine,Human,Mouse,Rabbit,Rat; observed MW: 70 kDa; calculated MW: 70176 MW.
Boster Bio Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22) catalog # MA1050. Tested in IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rabbit, Rat.
Key elements and design rationale
- Target: HSPA1A; HSPA1B — Heat shock 70 kDa protein 1B
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: BRM-22; Isotype: Mouse IgG1
- Species reactivity: Bovine,Human,Mouse,Rabbit,Rat
- Molecular weight guidance: Observed: 70 kDa; Calculated: 70176 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation. .
Scientific background (datasheet): Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex (MHC) class III region 92 kilobases (kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.
Cellular localization (datasheet): Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .
Tissue details (datasheet): Testis-specific.
Sequence similarities (datasheet): Belongs to the heat shock protein 70 family.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Chaperones,Heat Shock Proteins,Protein Trafficking,Signal Transduction,Tumor Biomarkers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
