| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heat shock 70 kDa protein 1B;Heat shock 70 kDa protein 1;HSP70.1;Hspa1b;Hcp70.1, Hsp70-1, Hsp70a1, Hspa1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | HSP70 isolated from bovine brain. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BRM-22 is a mouse monoclonal antibody that recognizes heat shock 70 kDa protein 1B (gene Hspa1b) in bovine, human, mouse, rabbit and rat samples and is validated for Western blot and IHC.
HSP70-1B (Hspa1b) is a stress-inducible HSP70 chaperone that, in mouse, is nearly identical to HSP70-1A. It uses ATP-dependent cycles of substrate binding and release to refold stressed proteins, prevent aggregation and target damaged proteins for degradation, and it is a common marker of the heat shock response.
| Target | Heat shock 70 kDa protein 1B (gene Hspa1b; UniProt P17879, mouse) |
|---|---|
| Clone | BRM-22 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Bovine, Human, Mouse, Rabbit, Rat |
| Purification | Ascites |
| Form | Lyophilized |
| Formulation | 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers |
| Reconstitution | 100 µg/mL after adding 1 mL PBS |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Bovine, Rabbit, Rat) |
|---|---|
| Western blot | 1 µg/mL (Human, Bovine, Mouse, Rabbit, Rat) |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
HSP70 isolated from bovine brain.
Reactivity Notes
The supplier lists reactivity with bovine, human, mouse, rabbit and rat. UniProt places heat shock 70 kDa protein 1B in the cytoplasm. Tissue expression noted by UniProt (mouse): Testis-specific.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Song Tingting et al. (2021) Dose-Effect of Zearalenone on the Localization and Expression of Growth Hormone, Growth Hormone Receptor, and Heat Shock Protein 70 in the Ovaries of Post-weaning Gilts. Frontiers in Veterinary Science. 10.3389/fvets.2021.629006
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Liyue Liu et al. (2013) Proteomic analysis of epithelioma papulosum cyprini cells infected with spring viremia of carp virus. Fish & Shellfish Immunology. 10.1016/j.fsi.2013.03.367
Bharati Jaya et al. (2016) Expression dynamics of HSP70 during chronic heat stress in Tharparkar cattle. International Journal of Biometeorology. 10.1007/s00484-016-1281-1
XUE-GANG XU et al. (2011) Immunohistological Evaluation of Skin Responses After Treatment Using a Fractional Ultrapulse Carbon Dioxide Laser on Back Skin. Dermatologic Surgery. 10.1111/j.1524-4725.2011.02062.x
Cui Naizhong et al. (2012) Effects of heat stress on the level of heat shock protein 70 on the surface of hepatocellular carcinoma Hep G2 cells: implications for the treatment of tumors. Tumor Biology. 10.1007/s13277-012-0603-0
