| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heat shock protein HSP 90-alpha; Heat shock 86 kDa; HSP 86; HSP86; Heat shock protein family C member 1; Lipopolysaccharide-associated protein 2; LAP-2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38; HSP90AA1; HSP90A; HSPC1; HSPCA |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp90 alpha, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 6B5 is a mouse monoclonal antibody that recognizes heat shock protein HSP 90-alpha (gene HSP90AA1) in human, mouse, rat and monkey samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 84.7 kDa, and the supplier reports an observed band at about 90 kDa.
HSP90α (HSP90AA1) is the stress-inducible form of the HSP90 molecular chaperone. It works through an ATP-driven cycle with many co-chaperones to fold, stabilize and activate client proteins such as kinases, steroid receptors and transcription factors. Because many cancer-related proteins depend on it, HSP90 is a major research and drug target.
| Target | Heat shock protein HSP 90-alpha (gene HSP90AA1; UniProt P07900, human) |
|---|---|
| Clone | 6B5 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human, Mouse, Rat, Monkey |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 84.7 kDa |
| Observed MW | 90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat, Monkey) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human HEK293 cells, monkey COS-7 cells, human HepG2 cells, human A549 cells, rat PC-12 cells, rat RH35 cells, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human cervical cancer tissue, human colon cancer tissue, human lung cancer tissue, human testis cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): SiHa cells.
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Synthetic peptide from the C-terminal region of human hsp90 alpha. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse, rat and monkey. UniProt places heat shock protein HSP 90-alpha in the nucleus, cytoplasm, melanosome and cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
