| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Multidrug resistance protein 1, ATP-binding cassette sub-family B (MDR/TAP) member 1, P-glycoprotein 1, P-gp, PGY1, ABC20, CD243, CLCS, Colchicin sensitivity, Doxorubicin resistance, GP170 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-Human ABCB1/MDR1 Antibody is an antibody targeting Multidrug resistance protein 1, ATP-binding cassette sub-family B (MDR/TAP) member 1, P-glycoprotein 1, P-gp, PGY1, ABC20, CD243, CLCS, Colchicin sensitivity, Doxorubicin resistance, GP170 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Multidrug resistance protein 1, ATP-binding cassette sub-family B (MDR/TAP) member 1, P-glycoprotein 1, P-gp, PGY1, ABC20, CD243, CLCS, Colchicin sensitivity, Doxorubicin resistance, GP170 (also reported as Multidrug resistance protein 1, ATP-binding cassette sub-family B (MDR/TAP) member 1, P-glycoprotein 1, P-gp, PGY1, ABC20, CD243, CLCS, Colchicin sensitivity, Doxorubicin resistance, GP170).
- Immunogen/epitope region: 3rd intracellular loop.
- Homology note: Not recommended for rat or mouse samples (informative for cross-species interpretation).
- Species reactivity (as provided): Human.
- Specificity statement (as provided): Not recommended for rat or mouse samples. The antibody is specific for MDR1. It won’t cross-react with the highly homologous MDR3 protein..
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Multidrug resistance protein 1 (MRP1, also known as P glycoprotein 1) is a member of a superfamily of proteins known as the ATP-binding cassette (ABC) transporters. This superfamiy is classified into seven subfamilies (A through G). Subfamily ABCC is composed of 12 proteins and includes MRP1 and is encoded by ABCC1.MRP1 structure comprises five domains with two nucleotide binding domains (NBDs) that bind ATP, and 12 transmembrane domains.MRP1 mediates the ATP-dependent efflux of a variety of solutes across membranes against a concentration gradient.
Research relevance and current trends
- Profiling immune-marker expression across cell subsets with single-cell or flow-based readouts.
- Connecting receptor/ligand levels to activation state and cytokine programs.
- Applying genetic perturbation or orthogonal assays to support specificity and interpretation.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-MT021.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-MT021; Negative control: BLP-MT021.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
