| Field | Specification |
|---|---|
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| Accession Number | |
| Alternative Names | C-C chemokine receptor type 2, Monocyte chemoattractant protein 1 receptor, MCP-1 receptor, CC-CKR-2, CD192 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
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| Target |
Overview
Anti-Human CCR2 (extracellular) Antibody is an antibody targeting C-C chemokine receptor type 2, Monocyte chemoattractant protein 1 receptor, MCP-1 receptor, CC-CKR-2, CD192 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IFC, LCI, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: C-C chemokine receptor type 2, Monocyte chemoattractant protein 1 receptor, MCP-1 receptor, CC-CKR-2, CD192 (also reported as C-C chemokine receptor type 2, Monocyte chemoattractant protein 1 receptor, MCP-1 receptor, CC-CKR-2, CD192).
- Immunogen/epitope region: Extracellular, N-terminus.
- Homology note: Human only (informative for cross-species interpretation).
- Species reactivity (as provided): Human.
- Specificity statement (as provided): The antibody will not recognize CCR2 from mouse and rat samples..
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
CC chemokine receptor 2 (CCR2) is one of 19 members of the chemokine receptor subfamily of class A G-protein-coupled receptors (GPCRs). CCR2 is expressed in monocytes, immature dendritic cells, and T-cell subpopulations, and mediates acute inflammation by driving leukocyte migration to damaged or infected tissues towards chemokine ligands such as CCL2. Elevated expression of chemokines and their receptors such as CCR2 and its ligands can contribute to chronic inflammation and malignancy and hence these molecules are implicated in numerous inflammatory and neurodegenerative diseases including atherosclerosis, multiple sclerosis, asthma, neuropathic pain, and diabetic nephropathy, as well as cancer.
Research relevance and current trends
- Profiling immune-marker expression across cell subsets with single-cell or flow-based readouts.
- Connecting receptor/ligand levels to activation state and cytokine programs.
- Applying genetic perturbation or orthogonal assays to support specificity and interpretation.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
- Live cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-CR022.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-CR022; Negative control: BLP-CR022.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
