| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Lysophosphatidic acid receptor 5, LPA-5, GPR92, GPR93 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-Human LPAR5 (extracellular) Antibody is an antibody targeting Lysophosphatidic acid receptor 5, LPA-5, GPR92, GPR93 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Lysophosphatidic acid receptor 5, LPA-5, GPR92, GPR93 (also reported as Lysophosphatidic acid receptor 5, LPA-5, GPR92, GPR93).
- Immunogen/epitope region: 3rd extracellular loop.
- Homology note: Mouse, Rat - not recommended for use with mouse and rat samples (informative for cross-species interpretation).
- Species reactivity (as provided): Human.
- Specificity statement (as provided): Will not recognize rat or mouse LPA5 receptor..
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
LPA (lysophosphatidic acid) mediates cellular differentiation, growth, survival and motility through two subgroups of G-protein coupled receptors (GPCRs): The Edg (endothelial differentiation gene) family - LPA1, LPA2, LPA3 and the non-Edg family - LPA4, LPA5, LPA61.Like all GPCRs, LPA receptors have seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail1.Alkyl-LPA, commonly found in platelets activates LPA5, thus explaining the high expression of the receptor in platelets2. LPA is expressed in astrocytes, and sensory and motor neurons in the spinal cord. It is also detected in spleen, heart, placenta, lliver and colon3.LPA5 activation leads to cAMP production; however, it does so in a Gs independent manner4,5.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-LR035.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-LR035; Negative control: BLP-LR035.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
