| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Mas-related G-protein coupled receptor member X2, MRGX2 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
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| Target |
Overview
Anti-Human MRGPRX2 (extracellular) Antibody is an antibody targeting Mas-related G-protein coupled receptor member X2, MRGX2 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IFC, LCI, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Mas-related G-protein coupled receptor member X2, MRGX2 (also reported as Mas-related G-protein coupled receptor member X2, MRGX2).
- Immunogen/epitope region: Extracellular, N-terminus..
- Homology note: Human only No cross reactivity with mouse or rat related proteins (informative for cross-species interpretation).
- Species reactivity (as provided): Human.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
MRGPRX2, also known as Mas-related G protein-coupled receptor member X2, is a member of the seven transmembrane G protein-coupled receptor (GPCR) superfamily and the Mas-related subfamily branch of the rhodopsin-like class A GPCRs or Mas-related G protein-coupled receptors (Mrgprs).1,2Mrgprs first described 25 years ago, comprise more than 50 receptors in humans and rodents, most of which are still considered orphan receptors, that is, receptors with no identified endogenous or synthetic ligands.1.2The MRGPRX subfamily consists of four proteins (MRGPRX1 to MRGPRX4). The four proteins have sequence similarity with the MrgA Mas-related subfamily, however, the MRGPRX genes are only present in primates with no true orthologues in mice or rats. 1,2MRGPRX2 highest expression is observed in mast cells, although it has also been identified in other peripheral blood cells, including basophils and eosinophils, and at low levels in other tissues, such as the gastrointestinal tract and the central nervous system.1.2The expression and function of MRGPRX2 in mast cells garnered considerable interest, as these cells play a crucial role in the immune system and are involved in allergic reactions and inflammation.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
- Live cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-MR072.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-MR072; Negative control: BLP-MR072.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
