| Field | Specification |
|---|---|
| Target | |
| Alternative names | Allograft inflammatory factor 1;AIF-1;Ionized calcium-binding adapter molecule 1;Protein G1;AIF1;G1, IBA1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Iba1, different from the related mouse sequence by seven amino acids, and from the related rat sequence by six amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets allograft inflammatory factor 1 (gene AIF1) in human, mouse, pig and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 16.7 kDa, and the supplier reports an observed band at about 17 kDa.
Allograft inflammatory factor 1 (AIF1, also called Iba1) is a calcium- and actin-binding protein expressed mainly in macrophages and microglia. It supports membrane ruffling, Rac signaling and phagocytosis, and Iba1 is widely used as a marker of microglia and macrophages in studies of inflammation and neuroinflammation.
| Target | Allograft inflammatory factor 1 (gene AIF1; UniProt P55008, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Pig, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 16.7 kDa |
| Observed MW | 17 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat, Pig) |
Samples with a confirmed band (WB): human HEL cells, human THP-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human spleen tissue, mouse brain tissue, rat brain tissue, pig brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human iba1. It differs from the mouse sequence at seven residues and from the rat sequence at six residues.
Reactivity Notes
The supplier lists reactivity with human, mouse, pig and rat. UniProt places allograft inflammatory factor 1 in the cytoplasm and cell projection. Tissue expression noted by UniProt (human): Detected in T-lymphocytes and peripheral blood mononuclear cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yan-Qing Guan et al. (2011) Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs. Biomaterials. 10.1016/j.biomaterials.2011.01.060
