| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon-induced transmembrane protein 1; Dispanin subfamily A member 2a; DSPA2a; Interferon-induced protein 17; Interferon-inducible protein 9-27; Leu-13 antigen; CD antigen CD225; IFITM1; CD225; IFI17 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human IFITM1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects interferon-induced transmembrane protein 1 (gene IFITM1) in human samples and is validated for Western blot. The predicted molecular weight is 14.0 kDa, and the supplier reports an observed band at about 14–17 kDa.
IFITM1 (interferon-induced transmembrane protein 1) is a small membrane protein that restricts entry of many enveloped viruses by blocking fusion of viral and cellular membranes. It localizes mainly to the plasma membrane and acts against a range of viruses, and it is studied in innate antiviral defense and interferon responses.
| Target | Interferon-induced transmembrane protein 1 (gene IFITM1; UniProt P13164, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 14.0 kDa |
| Observed MW | 14–17 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human K562 cells, human HeLa cells, human HepG2 cells, human Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human IFITM1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places interferon-induced transmembrane protein 1 in the cell membrane and lysosome membrane. Tissue expression noted by UniProt (human): Bone (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yang G et al. (2006) IFITM1 plays an essential role in the antiproliferative action of interferon-γ. Oncogene. 10.1038/sj.onc.1209807
Hatano Hiroko et al. (2008) IFN-Induced Transmembrane Protein 1 Promotes Invasion at Early Stage of Head and Neck Cancer Progression. Clinical Cancer Research. 10.1158/1078-0432.CCR-07-4761
Gun-Hoo Park et al. (2024) Interferon-Induced Transmembrane Protein 1 (IFITM1) Is Downregulated in Neurofibromatosis Type 1-Associated Malignant Peripheral Nerve Sheath Tumors. International Journal of Molecular Sciences. 10.3390/ijms25179265
