| Field | Specification |
|---|---|
| Target | |
| Alternative names | Inhibitor of nuclear factor kappa-B kinase subunit epsilon; I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IkBKE; Inducible I kappa-B kinase; IKK-i; IKKE; IKKI; KIAA0151 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human IKKi/IKKe Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFAO-9 is a rabbit monoclonal antibody that recognizes inhibitor of nuclear factor kappa-B kinase subunit epsilon (gene IKBKE) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 80.5 kDa, and the supplier reports an observed band at about 80 kDa.
IKKε (IKBKE, also called IKKi) is a non-canonical IκB kinase related to TBK1. After viral RNA or DNA sensing, it phosphorylates IRF3 and IRF7 to switch on type I interferon genes, and it also contributes to NF-κB and STAT signaling, linking it to antiviral immunity and inflammation.
| Target | Inhibitor of nuclear factor kappa-B kinase subunit epsilon (gene IKBKE; UniProt Q14164, human) |
|---|---|
| Clone | AFAO-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 80.5 kDa |
| Observed MW | 80 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human Raji cells, human Daudi cells, human Ramos cells, human HeLa cells, human THP-1 cells, human RT4 cells, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human IKKi/IKKe.
Reactivity Notes
The supplier lists reactivity with human. UniProt places inhibitor of nuclear factor kappa-B kinase subunit epsilon in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
