| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-15 receptor subunit alpha; IL-15 receptor subunit alpha; IL-15R-alpha; IL-15RA; CD antigen CD215; IL15RA |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human IL15RA |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27I41 is a rabbit monoclonal antibody that recognizes interleukin-15 receptor subunit alpha (gene IL15RA) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 28.2 kDa, and the supplier reports an observed band at about 38 kDa.
IL15RA is the high-affinity α chain of the interleukin-15 receptor. It binds IL-15 and typically presents it in trans to neighboring cells that express the IL-2/IL-15Rβ and common γ chains, a mechanism that supports NK cell and memory CD8+ T-cell homeostasis.
| Target | Interleukin-15 receptor subunit alpha (gene IL15RA; UniProt Q13261, human) |
|---|---|
| Clone | 27I41 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 28.2 kDa |
| Observed MW | 38 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human IL15RA.
Reactivity Notes
The supplier lists reactivity with human. UniProt places interleukin-15 receptor subunit alpha in the membrane, nuclear membrane and cell surface. Tissue expression noted by UniProt (human): Expressed in neutrophils (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
