Anti-IL1RA/Il1rn Antibody Picoband®

Overview

Anti-IL1RA/Il1rn Antibody Picoband® is an antibody for Il1rn detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: Il1rn (Interleukin-1 receptor antagonist protein); UniProt: P25085
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 18 kDa, calculated 20282 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-IL1RA/Il1rn Antibody Picoband® catalog # A00651-5.

Biological background

Biological context: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity (By similarity). .

Expression and localization notes: cellular localization: Secreted ., tissue context: The intracellular form of IL1RN is predominantly expressed in epithelial cells..

Common research applications

• Western blotting (WB): Compare Il1rn levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of Il1rn in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify Il1rn-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Secreted .
• Tissue details: The intracellular form of IL1RN is predominantly expressed in epithelial cells.
• Research category: Cytokines,Immunology,Innate Immunity,Interleukins

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.