| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-37; IL-37; FIL1 zeta; IL-1X; Interleukin-1 family member 7; IL-1F7; Interleukin-1 homolog 4; IL-1H; IL-1H4; Interleukin-1 zeta; IL-1 zeta; Interleukin-1-related protein; IL-1RP1; IL37; FIL1Z; IL1F7; IL1H4; IL1RP1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human IL37 recombinant protein (Position: V46-D218). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects interleukin-37 (gene IL37) in human samples and is validated for IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 24.1 kDa.
Interleukin-37 (IL-37, IL1F7) is an anti-inflammatory member of the IL-1 family with no mouse ortholog. It dampens innate immune responses both inside the cell, by moving to the nucleus with SMAD3, and outside the cell by binding IL-18Rα with the co-receptor IL-1R8. It lowers production of pro-inflammatory cytokines and is studied as a brake on excessive inflammation.
| Target | Interleukin-37 (gene IL37; UniProt Q9NZH6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 24.1 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with confirmed staining (IHC): human B lymphocytic tumor tissue, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): Raji cells.
Immunogen
Recombinant human IL37 fragment (Val46–Asp218), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes interleukin-37 as secreted and also located in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): In general, low constitutive expression, if any, in healthy tissues.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
