| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD antigen CD124; Il4r; Il4ra |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived rat IL4R recombinant protein (Position: I26-E719). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes interleukin-4 receptor subunit alpha (gene Il4r) in mouse and rat samples and is validated for IHC, flow cytometry and ELISA. The predicted molecular weight is 86.7 kDa.
IL-4Rα is the ligand-binding receptor chain shared by IL-4 and IL-13. Receptor engagement activates JAK kinases and STAT6, driving Th2 differentiation, IgE class switching and alternative activation of macrophages, and IL-4Rα is a therapeutic target in allergic disease.
| Target | Interleukin-4 receptor subunit alpha (gene Il4r; UniProt Q63257, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.005 mg NaN3 |
| Calculated MW | 86.7 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with confirmed staining (IHC): rat spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): mouse spleen tissue.
Immunogen
Recombinant rat IL4R fragment (Ile26–Glu719), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places interleukin-4 receptor subunit alpha in the cell membrane and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (rat): Isoform 2 is expressed in kidney, spleen, lung and liver.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
