| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Oncostatin-M; OSM; Osm |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived rat IL4R recombinant protein (Position: I26-E719). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-IL4R Antibody is an antibody for Il4r detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Il4r (oncostatin M); UniProt: Q63257
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 180 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-IL4R Antibody Picoband® catalog # A00807-2.
Biological background
Biological context: Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells (By similarity). Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
Expression and localization notes: cellular localization: Secreted., tissue context: Widely expressed. Expressed at higher levels in liver, skin and spleen..
Common research applications
- Western blotting (WB): Compare Il4r levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Il4r in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Il4r-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: IL4R (INTERLEUKIN 4 RECEPTOR) is a human gene that encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE antibody production in B cells. The IL4R gene is localized to 16p12.1-p11.2 by in situ hybridization and Southern blot analysis of DNA from a panel of mouse-human somatic cell lines. IL4 plays a major role in immunoglobulin E (IgE) production. Its signal is conferred to effector cells through binding to the alpha chain of the IL4 receptor. IL4 interacts with IL4R with high affinity, leading to dimerization with either the common gamma chain,a component of receptors for a number of cytokines, to create a type I receptor, or with IL13RA1 to form a type II receptor.The IL4R gene contains 12 exons and IL4R is a complex of at least 2 components, one of which is a novel affinity converting subunit that is critical for cellular signal transduction. See also IL13RA1 and IL13RA2.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Secreted.
- Tissue details: Widely expressed. Expressed at higher levels in liver, skin and spleen.
- Research category: Nuclear Signaling Pathways,Signal Transduction,Signaling Pathway
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
