| Field | Specification |
|---|---|
| Target | |
| Alternative names | Inhibin alpha chain; INHA |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Inhibin alpha |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22I25 is a rabbit monoclonal antibody that recognizes inhibin alpha chain (gene INHA) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 39.7 kDa, and the supplier reports an observed band at about 40 kDa.
The inhibin alpha subunit (INHA) pairs with a beta-A or beta-B subunit to form inhibin A or inhibin B, gonadal hormones made mainly by granulosa and Sertoli cells. Inhibins suppress pituitary FSH secretion by antagonizing activin signaling, and they are studied in reproductive endocrinology and gonadal function.
| Target | Inhibin alpha chain (gene INHA; UniProt P05111, human) |
|---|---|
| Clone | 22I25 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 39.7 kDa |
| Observed MW | 40 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human testis.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human inhibin alpha.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes inhibin alpha chain as a secreted protein. Tissue expression noted by UniProt (human): Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
