| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Nitric oxide synthase, inducible;1.14.13.39;Hepatocyte NOS;HEP-NOS;Inducible NO synthase;Inducible NOS;iNOS;N |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human iNOS |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-iNOS NOS2 Rabbit Monoclonal Antibody is an antibody targeting NOS2. Common applications include WB, ICC, IF, IP. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: IED-14; isotype: Rabbit IgG; reactivity: Human,Mouse; observed MW: 150 kDa; calculated MW: 131117 MW.
Boster Bio Anti-iNOS NOS2 Rabbit Monoclonal Antibody catalog # M00368. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: NOS2 — Nitric oxide synthase, inducible
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: IED-14; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse
- Molecular weight guidance: Observed: 150 kDa; Calculated: 131117 MW
Biological background
Protein function (datasheet): Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. As component of the iNOS- S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys- 247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. .
Cellular localization (datasheet): Cell membrane ; Single- pass type I membrane protein . The activated receptor is rapidly internalized and degraded.
Tissue details (datasheet): Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cancer Metabolism,Metabolism,Metabolism Processes,Neuroscience,Neurotransmission,Pathways and Processes,Response To Hypoxia.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
