| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin alpha-V; Vitronectin receptor; Vitronectin receptor subunit alpha; CD antigen CD51; ITGAV; MSK8; VNRA; VTNR |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Integrin alpha V/ITGAV recombinant protein (Position: H732-D970). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets integrin alpha-V (gene ITGAV) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 116.0 kDa, and the supplier reports an observed band at about 130–140 kDa.
Integrin alpha-V (ITGAV, CD51) pairs with several beta subunits (β1, β3, β5, β6, β8) to form receptors that recognize the RGD motif in vitronectin, fibronectin, osteopontin and other matrix proteins. Alpha-V integrins also activate latent TGF-β, and they are studied in angiogenesis, fibrosis and tumor biology.
| Target | Integrin alpha-V (gene ITGAV; UniProt P06756, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 116.0 kDa |
| Observed MW | 130–140 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A549 cells, human A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with a confirmed signal (flow cytometry): Raji cells.
Immunogen
Recombinant human Integrin alpha V/ITGAV fragment (His732–Asp970), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places integrin alpha-V in the cell membrane and cell junction. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qin Xing et al. (2016) TGFβ3-mediated induction of Periostin facilitates head and neck cancer growth and is associated with metastasis. Scientific Reports. 10.1038/srep20587
Yifei Wang et al. (2011) Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors. International Journal of Molecular Sciences. 10.3390/ijms12063409
Changzhi Wang et al. (2011) Overexpression of Lewis (y) Antigen Protects Ovarian Cancer RMG-1 Cells from Carboplatin-Induced Apoptosis by the Upregulation of Topo-I and Topo-II β. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology. 10.1002/ar.21398
