| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Voltage-dependent calcium channel subunit alpha-2/delta-2; Voltage-gated calcium channel subunit alpha-2/delta-2; Voltage-dependent calcium channel subunit alpha-2-2; Voltage-dependent calcium channel subunit delta-2; CACNA2D2; KIAA0558 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Integrin alpha V/ITGAV recombinant protein (Position: H732-D970). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Integrin alpha V/ITGAV Antibody Picoband® is an antibody for ITGAV detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: ITGAV (calcium voltage-gated channel auxiliary subunit alpha2delta 2); UniProt: P06756
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 130-140 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Integrin alpha V/ITGAV Antibody Picoband® catalog # A01561-2.
Biological background
Biological context: The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) and possibly T-type (CACNA1G). Overexpression induces apoptosis.
Expression and localization notes: cellular localization: Membrane; Colocalizes with CACNA1A in lipid raft fractions., tissue context: Predominantly present in cerebellar cortex. Present in various lung tumor cell lines, while it is absent in normal lung (at protein level). Highly expressed in heart, lung, testis, pancreas and skeletal muscle. Also expressed in kidney, liver, placenta and brain..
Common research applications
- Western blotting (WB): Compare ITGAV levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of ITGAV in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify ITGAV-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Integrin alpha-V is a protein that in humans is encoded by the ITGAV gene. It is a member of the beta 3 integrin subfamily (cytoadhesins). The human locus for the av gene (VNRA) was previously mapped to the long arm of chromosome 2. Sims et al. (2000) localized the VNRA gene to 2q31. The gene contains 30 exons and spans over 93 kb of genomic DNA. It functions as a receptor for a group of proteins that includes vitronectin, fibrinogen, thrombospondin, and von Willebrand factor.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Membrane; Colocalizes with CACNA1A in lipid raft fractions.
- Tissue details: Predominantly present in cerebellar cortex. Present in various lung tumor cell lines, while it is absent in normal lung (at protein level). Highly expressed in heart, lung, testis, pancreas and skeletal muscle. Also expressed in kidney, liver, placenta and brain.
- Research category: Apoptosis,Associated Proteins,Calcium Channels,Calcium Signaling,Cell Biology,Intracellular,Neuroscience,Neurotransmission,Signal Transduction,Signaling Pathway
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
