| Field | Specification |
|---|---|
| Target | |
| Alternative names | Inositol 1,4,5-trisphosphate-gated calcium channel ITPR1; IP3 receptor isoform 1; IP3R 1; InsP3R1; Inositol 1,4,5 trisphosphate receptor; Inositol 1,4,5-trisphosphate receptor type 1; Type 1 inositol 1,4,5-trisphosphate receptor; Type 1 InsP3 receptor; ITPR1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human IP3 Receptor |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17I50 is a rabbit monoclonal antibody that recognizes inositol 1,4,5-trisphosphate-gated calcium channel ITPR1 in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 313.9 kDa, and the supplier reports an observed band at about 314 kDa.
The inositol 1,4,5-trisphosphate receptor type 1 (IP3R1, ITPR1) is a calcium channel in the endoplasmic reticulum that opens when bound by IP3 and calcium. It is especially abundant in cerebellar Purkinje cells, and mutations in ITPR1 cause several forms of spinocerebellar ataxia. It is studied in calcium signaling and neurobiology.
| Target | Inositol 1,4,5-trisphosphate-gated calcium channel ITPR1 (gene ITPR1; UniProt Q14643, human) |
|---|---|
| Clone | 17I50 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 313.9 kDa |
| Observed MW | 314 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human SH-SY5Y cells, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human IP3 Receptor.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places inositol 1,4,5-trisphosphate-gated calcium channel ITPR1 in the endoplasmic reticulum membrane and cytoplasm. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lei Shi et al. (2023) Hyperoside ameliorates cerebral ischaemic–reperfusion injury by opening the TRPV4 channel in vivo through the IP3-PKC signalling pathway. Pharmaceutical Biology. 10.1080/13880209.2023.2228379
