| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | IQ Motif Containing GTPase Activating Protein 1, Ras GTPase-Activating-Like Protein IQGAP1, p195 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-IQGAP1 Antibody is an antibody targeting IQ Motif Containing GTPase Activating Protein 1, Ras GTPase-Activating-Like Protein IQGAP1, p195 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IF, IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: IQ Motif Containing GTPase Activating Protein 1, Ras GTPase-Activating-Like Protein IQGAP1, p195 (also reported as IQ Motif Containing GTPase Activating Protein 1, Ras GTPase-Activating-Like Protein IQGAP1, p195).
- Immunogen/epitope region: Intracellular.
- Homology note: Mouse, rat - 15/16 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
IQGAP1 is a 190 kDa (1657 amino acid) protein that is ubiquitously expressed and contains six distinct protein-interacting domains with six coiled coil regions1.The Ras GTPase-activating-like protein is a scaffold protein involved in regulating various cellular processes; from organizing the actin cytoskeleton, transcription, cell adhesion, and regulating the cell cycle2,3.IQGAP1 binds to activated CDC42 but does not stimulate its GTPase activity and it may have a role in cell cycle progression following DNA damage1,2. The protein also regulates NR2A NMDA receptor signaling where it influences cognitive processes4. It also regulates MAPK and Wnt/β-catenin signaling pathways5.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-PZ055.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-PZ055; Negative control: BLP-PZ055.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
