| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cystatin-B; CPI-B; Liver thiol proteinase inhibitor; Stefin-B; CSTB; CST6; STFB |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human KAT9/ELP3, identical to the related mouse sequence. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-KAT9/ELP3 Antibody Picoband® (monoclonal, 8B4) is an antibody for ELP3 detection raised in Mouse (Monoclonal, clone Clone: 8B4, Mouse IgG2a), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: ELP3 (cystatin B (stefin B)); UniProt: Q9H9T3
- Antibody format: Mouse, Monoclonal, clone Clone: 8B4, Mouse IgG2a
- Molecular weight: 62 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-KAT9/ELP3 Antibody Picoband® (monoclonal, 8B4) catalog # M02833-1.
Biological background
Biological context: This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
Expression and localization notes: cellular localization: Cytoplasm, tissue context: Expressed in all adult and fetal tissues except brain and skin. More abundant in fetal tissues especially liver..
Common research applications
- Western blotting (WB): Compare ELP3 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of ELP3 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify ELP3-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Elongator complex protein 3, also named KAT9, is aproteinthat in humans is encoded by theELP3gene. It is mapped to 8p21.1. ELP3 is the catalytichistone acetyltransferasesubunit of theRNA polymeraseII elongator complex, which is a component of the RNA polymerase II (Pol II)holoenzymeand is involved in transcriptional elongation. ELP3 supports the migration and branching ofprojection neuronsthroughacetylationof alpha-tubulin in the developingcerebral cortex. In mammals, ELP3 is important for paternalDNA demethylationafterfertilization.ELP3 is potentially involved in cellular redoxhomeostasisby mediating the acetylation ofglucose-6-phosphate dehydrogenase. Besides, ELP3 may play a role inchromatin remodelingand is involved in acetylation ofhistones H3and probablyH4.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytoplasm
- Tissue details: Expressed in all adult and fetal tissues except brain and skin. More abundant in fetal tissues especially liver.
- Research category: Cell Biology,Protease Inhibitors,Proteolysis/Ubiquitin
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
