| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | KCa4.1, KNa1.1, Slo2.2 Na+-activated K+ channel, Potassium channel subfamily T member 1 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-KCNT1 (Slack) Antibody is an antibody targeting KCa4.1, KNa1.1, Slo2.2 Na+-activated K+ channel, Potassium channel subfamily T member 1 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: KCa4.1, KNa1.1, Slo2.2 Na+-activated K+ channel, Potassium channel subfamily T member 1 (also reported as KCa4.1, KNa1.1, Slo2.2 Na+-activated K+ channel, Potassium channel subfamily T member 1).
- Immunogen/epitope region: Intracellular, C-terminus.
- Homology note: Mouse - 12/13 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Rat, Mouse.
- Specificity statement (as provided): Unlikely to recognize human samples..
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
KCa4.1 (Na+-activated K+ channel, Slack, Slo2.2, KCNT1) is a member of a subgroup within the Ca2+ activated K+ channel family. The Slack (Sequence like a Ca2+-activated K+ channel) and Slick (Sequence like an intermediate conductance K+ channel, KCNT2) genes, which encode Na+-activated K+ (KNa) channels, are expressed at high levels in neurons in several areas of the nervous system1.Although KCa4.1 is functionally a Na+-activated K+ channel, it is termed KCa by the IUPHAR nomenclature, due to its sequence homology to other KCa channels.The family's protein topology consists of six transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels, the functional unit for KCa4.1 channels is composed of four subunits that can assemble as either homo- or heteromers with KCNT2 (Slick).
Research relevance and current trends
- Linking transporter/channel abundance to ionic homeostasis and excitability-related phenotypes.
- Studying compartment-specific localization (surface vs intracellular pools) and trafficking dynamics.
- Combining antibody readouts with functional assays for more complete interpretation.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-PC124.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-PC124; Negative control: BLP-PC124.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
