| Field | Specification |
|---|---|
| Target | |
| Alternative names | Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67 homolog; Antigen Ki67 homolog; Mki67 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Mki67 recombinant protein (Position: F29-S3177). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes proliferation marker protein Ki-67 (gene Mki67) in human, mouse and rat samples and is validated for IHC and ELISA. The predicted molecular weight is 350.9 kDa.
Ki-67 (MKI67) is a large nuclear protein present in dividing cells but absent in resting (G0) cells. During mitosis it coats the chromosome surface and helps keep individual chromosomes separated, and because its expression tracks cell division, Ki-67 is one of the most widely used markers of cell proliferation.
| Target | Proliferation marker protein Ki-67 (gene Mki67; UniProt E9PVX6, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 350.9 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with confirmed staining (IHC): human tonsil tissue, human lung cancer tissue, human thyroid cancer tissue, mouse thymus tissue, rat thymus tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse Mki67 fragment (Phe29–Ser3177), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places proliferation marker protein Ki-67 in the chromosome and nucleus. Tissue expression noted by UniProt (mouse): Mainly present in proliferating cells (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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