| Field | Specification |
|---|---|
| Target | |
| Alternative names | Kininogen-1;Alpha-2-thiol proteinase inhibitor;Fitzgerald factor;High molecular weight kininogen;HMWK;Williams-Fitzgerald-Flaujeac factor;Kininogen-1 heavy chain;T-kinin;Ile-Ser-Bradykinin;Bradykinin;Kallidin I;Lysyl-bradykinin;Kallidin II;Kininogen-1 light chain;Low molecular weight growth-promoting factor;KNG1;BDK, KNG; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Kininogen 1 recombinant protein (Position: Q19-N210). Human Kininogen 1 shares 63% and 66% amino acid (aa) sequence identity with mouse and rat Kininogen 1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects kininogen-1 (gene KNG1) in human samples and is validated for Western blot. The predicted molecular weight is 72.0 kDa, and the supplier reports an observed band at about 72 kDa.
Kininogen-1 (KNG1) is a plasma protein made in the liver and produced as high- and low-molecular-weight forms. High-molecular-weight kininogen acts as a cofactor in the contact pathway of coagulation, and cleavage of kininogens by kallikreins releases bradykinin or kallidin, peptides that dilate blood vessels and increase vascular permeability. Kininogens also inhibit cysteine proteases.
| Target | Kininogen-1 (gene KNG1; UniProt P01042, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 72.0 kDa |
| Observed MW | 72 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human placenta tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human Kininogen 1 fragment (Gln19–Asn210), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 63% and 66%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes kininogen-1 as a secreted protein. Tissue expression noted by UniProt (human): Secreted in plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
