| Field | Specification |
|---|---|
| Target | |
| Alternative names | Krueppel-like factor 6;B-cell-derived protein 1;Core promoter element-binding protein;GC-rich sites-binding factor GBF;Proto-oncogene BCD1;Suppressor of tumorigenicity 12 protein;Transcription factor Zf9;KLF6;BCD1, COPEB, CPBP, ST12; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human KLF6 recombinant protein (Position: E38-N205). Human KLF6 shares 92% and 90% amino acid (aa) sequences identity with mouse and rat KLF6, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes krueppel-like factor 6 (gene KLF6) in human and rat samples and is validated for Western blot. The predicted molecular weight is 31.9 kDa, and the supplier reports an observed band at about 37 kDa.
KLF6 is a Krüppel-like zinc finger transcription factor that binds GC-box elements and generally activates its targets. It is involved in cell differentiation, growth control and tissue injury responses, and it can promote cell cycle arrest, for example by inducing p21. KLF6 has been described as a tumor suppressor in several cancers and is studied in liver fibrosis.
| Target | Krueppel-like factor 6 (gene KLF6; UniProt Q99612, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 31.9 kDa |
| Observed MW | 37 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): human placenta tissue, rat testis tissue, HeLa cells, HepG2 cells, HEPA cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant human KLF6 fragment (Glu38–Asn205), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 92% and 90%, respectively.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places krueppel-like factor 6 in the nucleus. Tissue expression noted by UniProt (human): Highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
