| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Krueppel-like factor 6;B-cell-derived protein 1;Core promoter element-binding protein;GC-rich sites-binding factor GBF;Proto-oncogene BCD1;Suppressor of tumorigenicity 12 protein;Transcription factor Zf9;KLF6;BCD1, COPEB, CPBP, ST12; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human KLF6 recombinant protein (Position: E38-N205). Human KLF6 shares 92% and 90% amino acid (aa) sequences identity with mouse and rat KLF6, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of KLF6 (Krueppel-like factor 6) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-KLF6 Antibody Picoband® catalog # PB9231. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human KLF6 recombinant protein (Position: E38-N205). Human KLF6 shares 92% and 90% amino acid (aa) sequences identity with mouse and rat KLF6, respectively. (reported region: E38-N205).
- Molecular weight context: reported MW: 37 kDa; calculated MW: 31865 MW
- Reactivity: Human,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Krueppel-like factor 6; Krueppel-like factor 6. Krueppel-like factor 6 (KLF6) is a protein that in humans is encoded by the KLF6 gene. It is a tumor suppressor gene which is located on 10p15.1. This gene encodes a nuclear protein that has three zinc fingers at the end of its C-terminal domain, a serine/threonine-rich central region, and an acidic domain lying within the N-terminal region. The zinc fingers of this protein are responsible for the specific DNA binding with the guanine-rich core promoter elements. The central region might be involved in activation or posttranslational regulatory pathways, and the acidic N-terminal domain might play an important role in the process of transcriptional activation. It is capable of activating transcription approximately 4-fold either on homologous or heterologous promoters. The DNA binding and transcriptional activity of this protein, in conjunction with its expression pattern, suggests that this protein may participate in the regulation and/or maintenance of the basal expression of pregnancy-specific glycoprotein genes and possibly other TATA box-less genes. Functional note: Transcriptional activator (By similarity). Binds a GC box motif. Could play a role in B-cell growth and development. . Reported localization: Nucleus. Expression/tissue context: Highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. Weakly expressed in pancreas, lung, liver, heart and skeletal muscle. Also expressed in fetal brain, spleen and thymus.
Research relevance and current trends
- Developmental Families: Researchers commonly examine how KLF6 (Krueppel-like factor 6) relates to this theme using model systems and orthogonal readouts.
- Domain Families: Researchers commonly examine how KLF6 (Krueppel-like factor 6) relates to this theme using model systems and orthogonal readouts.
- Epigenetics and Nuclear Signaling: Researchers commonly examine how KLF6 (Krueppel-like factor 6) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative KLF6 (Krueppel-like factor 6) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the krueppel C2H2-type zinc-finger protein family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
