| Field | Specification |
|---|---|
| Target | |
| Alternative names | Krueppel-like factor 3;Basic krueppel-like factor;CACCC-box-binding protein BKLF;TEF-2;KLF3;BKLF; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human KLF3, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes krueppel-like factor 3 (gene KLF3) in rat, mouse and human samples and is validated for Western blot. The predicted molecular weight is 38.8 kDa, and the supplier reports an observed band at about 18 kDa.
KLF3 (BKLF) is a Krüppel-like zinc finger transcription factor that binds CACCC-box elements. It mainly represses genes by recruiting the corepressor CtBP and is highly expressed in the erythroid lineage, where it helps control red blood cell gene expression. KLF3 is also studied in fat cell and B-cell development.
| Target | Krueppel-like factor 3 (gene KLF3; UniProt P57682, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat, Mouse, Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 38.8 kDa |
| Observed MW | 18 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat, Mouse) |
|---|
Samples with a confirmed band (WB): rat testis tissue, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the N-terminal region of human KLF3. It differs from the rat sequence at one residue and from the mouse sequence at two residues.
Reactivity Notes
The supplier lists reactivity with rat, mouse and human. UniProt places krueppel-like factor 3 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
