Anti-Ku70 XRCC6 Rabbit Monoclonal Antibody

Overview

Anti-Ku70 XRCC6 Rabbit Monoclonal Antibody is an antibody targeting XRCC6. Common applications include WB, IHC, ICC, IF, IP, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AAIG-24; isotype: Rabbit IgG; reactivity: Human; observed MW: 68 kDa; calculated MW: 69843 MW.

Boster Bio Anti-Ku70 XRCC6 Rabbit Monoclonal Antibody catalog # M01732-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Key elements and design rationale

• Target: XRCC6 — X-ray repair cross-complementing protein 6
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AAIG-24; Isotype: Rabbit IgG
• Species reactivity: Human
• Molecular weight guidance: Observed: 68 kDa; Calculated: 69843 MW

Biological background

Protein function (datasheet): Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' ion. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V (D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose- 5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. .

Cellular localization (datasheet): Nucleus . Chromosome .

Tissue details (datasheet): Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level). .

Research relevance and current trends

• Commonly studied in contexts related to DNA/RNA,DNA Damage & Repair,Epigenetics and Nuclear Signaling.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
• Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
• Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.