| Field | Specification |
|---|---|
| Target | |
| Alternative names | Laminin subunit alpha-3;Epiligrin 170 kDa subunit;E170;Epiligrin subunit alpha;Kalinin subunit alpha;Laminin-5 subunit alpha;Laminin-6 subunit alpha;Laminin-7 subunit alpha;Nicein subunit alpha;LAMA3;LAMNA; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human LAMA3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOAB-12 is a rabbit monoclonal antibody that recognizes laminin subunit alpha-3 (gene LAMA3) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 366.6 kDa, and the supplier reports an observed band at about 367 kDa.
Laminin alpha-3 (LAMA3) is a chain of laminin-332 (laminin-5), a key component of epithelial basement membranes. Laminin-332 links basal keratinocytes to the underlying matrix through integrin alpha-3/beta-1 and alpha-6/beta-4 in hemidesmosomes and supports cell adhesion, migration and differentiation. Mutations in LAMA3 cause junctional epidermolysis bullosa.
| Target | Laminin subunit alpha-3 (gene LAMA3; UniProt Q16787, human) |
|---|---|
| Clone | AOAB-12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 366.6 kDa |
| Observed MW | 367 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human LAMA3.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Skin. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhongxi Sun et al. (2026) A chitosan-based thermosensitive hydrogel incorporating propranolol nanoparticles to promote early osseointegration and peri-implant soft-tissue healing. RSC Advances. 10.1039/D6RA00103C
