| Field | Specification |
|---|---|
| Target | |
| Alternative names | Microtubule-associated proteins 1A/1B light chain 3B;Autophagy-related protein LC3 B;Autophagy-related ubiquitin-like modifier LC3 B;MAP1 light chain 3-like protein 2;MAP1A/MAP1B light chain 3 B;MAP1A/MAP1B LC3 B;Microtubule-associated protein 1 light chain 3 beta;MAP1LC3B;MAP1ALC3; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human LC3B |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IEC-13 is a rabbit monoclonal antibody that recognizes microtubule-associated protein 1 light chain 3 beta (gene MAP1LC3B) in human, mouse and rat samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 14.7 kDa; the supplier reports observed bands at 15, 18 kDa.
LC3B (MAP1LC3B) is a ubiquitin-like protein of the ATG8 family that is lipidated and anchored to autophagosome membranes. It helps expand the phagophore and binds cargo receptors such as p62 in selective autophagy, including mitophagy, and the conversion of LC3-I to lipidated LC3-II is the most widely used readout of autophagy in Western blot and imaging studies.
| Target | Microtubule-associated protein 1 light chain 3 beta (gene MAP1LC3B; UniProt Q9GZQ8, human) |
|---|---|
| Clone | IEC-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 14.7 kDa |
| Observed MW | 15, 18 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human A549 cells, human U2OS cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse Neurao-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human LC3B.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places microtubule-associated protein 1 light chain 3 beta in the endomembrane system, mitochondrion membrane, cytoplasm and cytoplasmic vesicle. Tissue expression noted by UniProt (human): Most abundant in heart, brain, skeletal muscle and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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