| Field | Specification |
|---|---|
| Target | |
| Alternative names | L-lactate dehydrogenase A chain;LDH-A;1.1.1.27;Cell proliferation-inducing gene 19 protein;LDH muscle subunit;LDH-M;Renal carcinoma antigen NY-REN-59;LDHA;PIG19; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human LDHA recombinant protein (Position: A2-R106). Human LDHA shares 94.3% amino acid (aa) sequence identity with both mouse and rat LDHA. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes L-lactate dehydrogenase A chain (gene LDHA) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 36.7 kDa, and the supplier reports an observed band at about 37 kDa.
Lactate dehydrogenase A (LDHA, LDH-M) is the muscle-type subunit of lactate dehydrogenase, a tetrameric enzyme that interconverts pyruvate and lactate while cycling NADH and NAD+. LDHA favors lactate production, supporting glycolysis when oxygen is limited and in rapidly dividing cells, so it is widely studied in cancer metabolism, the Warburg effect and hypoxia.
| Target | L-lactate dehydrogenase A chain (gene LDHA; UniProt P00338, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 36.7 kDa |
| Observed MW | 37 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human 293T cells, human MCF-7 cells, human A375 cells, human HepG2 cells, rat liver tissue, rat RH35 cells, mouse liver tissue, human MSTO-211H- WT cells, human MSTO-211H-LDHA KO cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue, rat intestine tissue, human mammary cancer tissue, mouse skeletal tissue, rat muscle skeletal tissue, human stomach cancer tissue, human thyroid cancer tissue, human pancreas cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with confirmed staining (ICC/IF): mouse muscle skeletal tissue, rat muscle skeletal tissue, human liver cancer tissue, human stomach cancer tissue, human pancreas cancer tissue.
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Recombinant human LDHA fragment (Ala2–Arg106), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 94.3%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places L-lactate dehydrogenase A chain in the cytoplasm. Tissue expression noted by UniProt (human): Predominantly expressed in anaerobic tissues such as skeletal muscle and liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiaodong Zou et al. (2022) Lactate facilitates classical swine fever virus replication by enhancing cholesterol biosynthesis. iScience. 10.1016/j.isci.2022.105353
Long Niya et al. (2020) CircPOSTN/miR-361-5p/TPX2 axis regulates cell growth, apoptosis and aerobic glycolysis in glioma cells. Cancer Cell International. 10.1186/s12935-020-01454-x
Xiaoyan Jiang et al. (2021) Lipopolysaccharide Affects the Proliferation and Glucose Metabolism of Cervical Cancer Cells Through the FRA1/MDM2/p53 Pathway. International Journal of Medical Sciences. 10.7150/ijms.47360
Yan Huang et al. (2024) Lactate dehydrogenase A is a diagnostic biomarker associated with immune infiltration, m6A modification and ferroptosis in endometrial cancer. Frontiers in Oncology. 10.3389/fonc.2024.1458344
Shiwei Yang et al. (2025) Skullcapflavone II Suppresses Colorectal Cancer by Inhibiting the HIF-1α-Glycolysis Pathway. Chemical Biology & Drug Design. 10.1111/cbdd.70184
Madhulika Rai et al. (2024) Characterization of genetic and molecular tools for studying the endogenous expression of Lactate dehydrogenase in Drosophila melanogaster. PLoS One. 10.1371/journal.pone.0287865
Jiaqi Liu et al. (2026) Effect and Mechanism of Shenqi Lixin Formula on Angiogenesis and Glucose Metabolism in Rats With Heart Failure After Myocardial Infarction. Clinical and Experimental Pharmacology and Physiology. 10.1111/1440-1681.70100
Hong-Peng Tian et al. (2025) Impact of SLC16A8 on tumor microenvironment and angiogenesis in colorectal cancer: New therapeutic target insights. World Journal of Gastrointestinal Oncology. 10.4251/wjgo.v17.i4.99188
