| Field | Specification |
|---|---|
| Target | |
| Alternative names | Low-density lipoprotein receptor;LDL receptor;LDLR; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human LDL Receptor |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BFC-12 is a rabbit monoclonal antibody that recognizes low-density lipoprotein receptor (gene LDLR) in human and mouse samples and is validated for Western blot and IHC. The predicted molecular weight is 95.4 kDa, and the supplier reports an observed band at about 140 kDa.
The LDL receptor (LDLR) is a cell surface receptor that binds apolipoprotein B- and E-containing lipoproteins, above all LDL, and carries them into cells by clathrin-mediated endocytosis. Hepatic LDLR is the main route for clearing LDL cholesterol from plasma, and LDLR mutations cause familial hypercholesterolemia. It is central to lipid metabolism and cardiovascular research, including studies of PCSK9.
| Target | Low-density lipoprotein receptor (gene LDLR; UniProt P01130, human) |
|---|---|
| Clone | BFC-12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 95.4 kDa |
| Observed MW | 140 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human liver carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human LDL Receptor.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places low-density lipoprotein receptor in the cell membrane, membrane, Golgi apparatus and early endosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
